miR-320a/SP1 negative reciprocal interaction contributes to cell growth and invasion in colorectal cancer

Abstract Background Transcription factors (TFs) may be engaged in reciprocal regulatory circuits with certain miRNAs to maintain cellular homeostasis. Disequilibrium of the reciprocities by tumor-related stimuli give rise deregulation downstream signaling pathways, thus promoting malignant tumor phenotypes. Specificity Protein 1 (SP1) is most representative member transcription factors. Previous studies disclosed that SP1 can transcriptionally regulate and coding genes facilitate progression. In our study, we used bioinformatic analysis predict several SP1-binding sites within miR-320a promoter found a predicted target gene miR-320a. Therefore, hypothesize link between participates colorectal cancer (CRC) development Methods We performed analysis, quantitative polymerase chain reaction (qPCR), immunoblotting, dual-luciferase reporter assays, series vitro vivo functional assays describe novel SP1/miR-320a interaction CRC Results First, was significantly downregulated tissues cell lines. Consistent findings other cancers, exhibited inhibitory effects on growth invasion vivo. Moreover, identified as miR-320a, ectopic expression partly abolished miR-320a-induced effects. Conversely, confirmed interacts promoter, leading depression This illustrates double-negative feedback loop SP1. Additionally, based fact promotes MACC1 transcription, determined via immunoblotting oncogenic MACC1/MET pathway inactivated context downregulation Conclusion Taken together, study first miR-320a/SP1 negative interaction, which contributes through modulation pathway.